Tag

Part:BBa_K2586004:Design

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-11)


Strep-Tag-aroE: aroE linked to a Strep-Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1390
    Illegal EcoRI site found at 74
    Illegal PstI site found at 1232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 74
    Illegal NheI site found at 24
    Illegal NheI site found at 192
    Illegal SpeI site found at 1391
    Illegal PstI site found at 1232
    Illegal PstI site found at 1405
    Illegal NotI site found at 7
    Illegal NotI site found at 1398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 74
    Illegal BamHI site found at 95
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1391
    Illegal EcoRI site found at 74
    Illegal PstI site found at 1232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 74
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1391
    Illegal PstI site found at 1232
    Illegal PstI site found at 1405
    Illegal NgoMIV site found at 517
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60


Design Notes

We added the Strep-Tag to (BBa_K2586003) to study protein-protein interactions of the EPSP-Synthase, which is encoded by the aroE gene.

Source

This part used (BBa_K2586003) as source.

References

(Herzberg et. al. 2007) : SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics. 7:4032-4035